Isothermal titration calorimetry is a physical technique used to measure the heat change that occurs when two molecules interact with each other. It can be used to characterize the interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules, but also to measure enzyme kinetics.
The MicroCal PEAQ-ITC Manual is an easy-to-use instrument with minimal sample preparation. As it is a label-free in solution technique, it ensures the analysis of unaltered biomolecules in their native state over a broad dynamic range. Dissociation constants can be measured down to 10-12 M (using direct or competitive binding techniques). The instrument operates in a temperature range between 2-80 °C.